- Jay Gibbons (Manitowoc 96-97) is starting the year as the Orioles leftfielder but will see plenty of time as the DH this season.
- Robb Quinlan (Dubuque, St. Cloud 96, 98) should see time at 1B and 3B this season for the Angels.
- Curtis Granderson (Mankato 01) is the starting centerfielder and the leadoff hitter for the Tigers.
- Juan Pierre (Manitowoc 96) is starting in centerfield for the Dodgers this year.
- Bobby Kielty (Kenosha 96) is a backup outfielder with the Athletics.
- Jay Marshall (Rochester 02) went from A ball last season to the Oakland bullpen this season.
- Jeff Weaver (Dubuque 95) is in the Mariners starting rotation this year.
- George Sherrill (Kenosha 97-98) is in the Seattle bullpen this season.
- Josh Willingham (Waterloo 00) is the starting leftfielder for the Marlins this year.
- Jeremy Accardo (Alexandria 01) is in the Toronto bullpen this season.
- Casey Janssen (Wisconsin 01) also is in the bullpen for the Bluejays this year.
- Paul McAnulty (Mankato 01) will be backing up 1B, 3B, and the OF for the Padres this season.
- Tom Gorzelanny (St. Cloud 02) has earned a spot in the starting rotation for the Pirates this year.
- Andre Ethier (Rochester 02) is starting in rightfield for the Dodgers this season.
- Mike Rouse (Brainerd 99) is backing up the infield positions this season for the Indians.
- Ben Zobrist (Wisconsin 03) is starting at shortstop for the Devil Rays this year.
- Pat Neshek (Wisconsin 02) will be in the Minnesota bullpen this season.
